human fibroblast growth factor 10  (R&D Systems)

Journal: Cellular and Molecular Gastroenterology and Hepatology

Article Title: Helicobacter pylori -Induced Inflammatory Cancer Transformation Microenvironment Drives Gastric Carcinogenesis

doi: 10.1016/j.jcmgh.2026.101757

Figure Lengend Snippet: Validation of the pro-tumorigenic potential of H pylori -driven fibroblasts from cell line to organoid models. ( A ) Enhanced stemness (sphere formation assay) and metastatic capacity (Transwell migration or invasion assays) in GES1 cells treated with recombinant C3 or CCN2 vs the control (quantification via bar plots; unpaired t -test). ( B ) Malignant phenotype induction in GES1 exposed to H pylori post-treated CM ( H pylori -treated CM) vs the control CM (sphere formation and Transwell assays; unpaired t -test quantified). ( C ) Schematic of gastric epithelial organoid-gastric fibroblast co-culture system. ( D ) Representative bright-field images of organoids co-cultured with NFs or H pylori -stimulated fibroblasts (NF-Hp) from Day 1 to 6 (3 replicates per group, upper; scale bar = 100 μm). Lower panel : statistics of median diameter (Day 1–6, organoids with diameter > 50 μm). ( E ) H&E staining ( left ) and immunofluorescence staining ( right ) of organoids in each group; markers: MUC5AC ( red ), CLDN7 ( green ), DAPI ( blue ). Statistics of MUC5AC-positive and CLDN7-positive area percentages (lower, ∗∗ P < .01; ∗∗∗∗ P < .0001).

Article Snippet: Exactly 5000 viable cells per well were seeded in sphere formation medium consisting of DMEM/F12 supplemented with 2% B27 Supplement, 20 ng/mL recombinant human epidermal growth factor (EGF), and 10 ng/mL recombinant human fibroblast growth factor (FGF) (all Gibco).

Techniques: Biomarker Discovery, Tube Formation Assay, Migration, Recombinant, Control, Co-Culture Assay, Cell Culture, Staining, Immunofluorescence

Journal: Biomedicines

Article Title: Growth Factor-Primed WJ-MSC Secretome Enhances Fibroblast Expansion In Vitro

doi: 10.3390/biomedicines13122863

Figure Lengend Snippet: Analysis of cytotoxicity, metabolic activity, and proliferation in human dermal fibroblasts cultured with the secretome of WJ-MSCs. ( a ) Live and dead assay. Calcein green fluorescent stain shows live cells, and ethidium homodimer red fluorescent stain denotes dead cells. ( b ) Prestoblue colorimetric assay. Metabolic activity was measured at 24, 72, and 168 h. At 24 h, S1.25 did not differ significantly from FBS or GFs controls, whereas 1.25, 6.25, and S6.25 were significantly lower (* p < 0.03, ** p < 0.01, and *** p < 0.008). At 72 h, S1.25 remained comparable to FBS but differed from GFs ( p < 0.04) and from 1.25, 6.25, and S6.25 ( p < 0.03). After 168 h, FBS showed the highest cell density, significantly exceeding that of all groups. ( c ) Histograms of CFSE in human fibroblasts with different concentrations of WJ-MSC secretome obtained from cells stimulated and unstimulated with FGF-EGF. ( c’ ) MIF graphic of CFSE. The graphic shows that FBS has lower MIF levels, indicating more cell divisions. GFs and S1.25 exhibited similar patterns in their MIF. * p < 0.04 and ** p < 0.01). n = 3 independent experiments, each performed in triplicate. Bars = 50 μm.

Article Snippet: Human fibroblasts (2 × 10 5 ) were cultured in T25 flasks with DMEM supplemented with 10% FBS and 1% antibiotic (penicillin, streptomycin and amphotericin B, Gibco, Waltham, MA, USA) and exposed either to secretome from stimulated WJ-MSCs (1.25 or 6.25 mg/mL), secretome from unstimulated WJ-MSCs (S1.25 or S6.25 mg/mL), or to exogenous GFs (EGF and FGF).

Techniques: Activity Assay, Cell Culture, Staining, Colorimetric Assay

Journal: Biomedicines

Article Title: Growth Factor-Primed WJ-MSC Secretome Enhances Fibroblast Expansion In Vitro

doi: 10.3390/biomedicines13122863

Figure Lengend Snippet: Scratch wound assay on fibroblast cultured with WJ-MSC secretome. ( a ) DIC photomicrographs of fibroblast cultures at day 0 and day 4 after scratching. ( b ) Quantification of scratch area occupied by fibroblasts expressed as a percentage, (*** p < 0.001, ** p < 0.01, * p < 0.05). n = 3 independent experiments, each performed in triplicate. Bars = 50 μm.

Article Snippet: Human fibroblasts (2 × 10 5 ) were cultured in T25 flasks with DMEM supplemented with 10% FBS and 1% antibiotic (penicillin, streptomycin and amphotericin B, Gibco, Waltham, MA, USA) and exposed either to secretome from stimulated WJ-MSCs (1.25 or 6.25 mg/mL), secretome from unstimulated WJ-MSCs (S1.25 or S6.25 mg/mL), or to exogenous GFs (EGF and FGF).

Techniques: Scratch Wound Assay Assay, Cell Culture

Journal: Biomedicines

Article Title: Growth Factor-Primed WJ-MSC Secretome Enhances Fibroblast Expansion In Vitro

doi: 10.3390/biomedicines13122863

Figure Lengend Snippet: Procollagen expression on fibroblasts cultured with WJ-MSC secretome. ( a ) Immunohistochemistry demonstration of procollagen-I after 7 days of culture. ( b ) Histograms of Procollagen marked in human fibroblasts with different concentrations of WJ-MSC secretome. ( c ) Graphic of MIF procollagen I expression after 7 days of culture. Flow cytometry revealed that S6.25 showed a significantly expression of procollagen than other experimental groups (* = p < 0.01). n = 3 independent experiments, each performed in triplicate. Bars = 50 μm.

Article Snippet: Human fibroblasts (2 × 10 5 ) were cultured in T25 flasks with DMEM supplemented with 10% FBS and 1% antibiotic (penicillin, streptomycin and amphotericin B, Gibco, Waltham, MA, USA) and exposed either to secretome from stimulated WJ-MSCs (1.25 or 6.25 mg/mL), secretome from unstimulated WJ-MSCs (S1.25 or S6.25 mg/mL), or to exogenous GFs (EGF and FGF).

Techniques: Expressing, Cell Culture, Immunohistochemistry, Flow Cytometry

Journal: Biomedicines

Article Title: Growth Factor-Primed WJ-MSC Secretome Enhances Fibroblast Expansion In Vitro

doi: 10.3390/biomedicines13122863

Figure Lengend Snippet: Alpha actin expression on fibroblasts cultured with WJ-MSC secretome. ( a ) Immunohistochemistry demonstration of alpha actin after 7 days of culture. ( b ) Histograms of alpha actin marked in human fibroblasts with different concentrations of WJ-MSC secretome. ( c ) Graphic of MIF alpha actin expression after 7 days of culture. The lowest alpha-actin expression was present in FBS group (*** p < 0.001) whereas S1.25 and 6.25 groups showed a higher expression (** = p < 0.01). n = 3 independent experiments, each performed in triplicate.

Article Snippet: Human fibroblasts (2 × 10 5 ) were cultured in T25 flasks with DMEM supplemented with 10% FBS and 1% antibiotic (penicillin, streptomycin and amphotericin B, Gibco, Waltham, MA, USA) and exposed either to secretome from stimulated WJ-MSCs (1.25 or 6.25 mg/mL), secretome from unstimulated WJ-MSCs (S1.25 or S6.25 mg/mL), or to exogenous GFs (EGF and FGF).

Techniques: Expressing, Cell Culture, Immunohistochemistry